山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Flexibacter Medium

培養(yǎng)基配方
Composition per liter:
Agar......................................................................15.0g
Tryptone...................................................................5.0g
Yeast extract..............................................................5.0g
Tris(hydroxymethyl)aminomethane buffer.....................................1.0g
KNO3 ......................................................................0.5g
Sodium β-glycerophosphate.................................................0.1g
Seawater...................................................................1.0L
Trace elements solution HO-LE.............................................1.0mL
pH 7.0 ± 0.2 at 25°C
Trace Elements Solution HO-LE:
Composition per liter:
H3BO3 ....................................................................2.85g
MnCl2 ·4H2O ..............................................................1.8g
Sodium tartrate...........................................................1.77g
FeSO4 ·7H2O..............................................................1.36g
CoCl2 ·6H2O .............................................................0.04g
CuCl2· 2H2O.............................................................0.027g
Na2MoO4 ·2H2O ..........................................................0.025g
ZnCl2 ...................................................................0.020g
Preparation of Trace Elements Solution HO-LE: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components to filtered, aged seawa-
ter and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or
flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation and maintenance of Cytophaga latercula, Flexi-
bacter aurantiacus, Flexibacter flexilis, Flexibacter roseolus, Flexibacter
ruber, Flexithrix dorotheae, and Saprospira grandis.