山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Flexibacter Medium

培養(yǎng)基配方
Composition per liter:
Agar....................................................................15.0g
Tris(hydroxymethyl)aminomethane buffer...................................1.0g
Casamino acids...........................................................1.0g
MgSO4 ·7H2O.............................................................0.1g
KNO3 ....................................................................0.1g
CaCl2 ·2H2O ............................................................0.1g
Sodium β-glycerophosphate...............................................0.1g
Thiamine................................................................1.0mg
Cobalamin...............................................................1.0μg
Glucose solution .......................................................10.0mL
Trace elements solution HO-LE............................................1.0mL
pH 7.5 ± 0.2 at 25°C
Glucose Solution:
Composition per 10.0mL:
D- Glucose..............................................................1.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Trace Elements Solution HO-LE:
Composition per liter:
H3BO3 ..................................................................2.85g
MnCl2 ·4H2O ............................................................1.8g
Sodium tartrate.........................................................1.77g
FeSO4 ·7H2O............................................................1.36g
CoCl2 ·6H2O............................................................0.04g
CuCl2· 2H2O...........................................................0.027g
Na2MoO4 ·2H2O.........................................................0.025g
ZnCl2 .................................................................0.020g
Preparation of Trace Elements Solution HO-LE: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except glucose solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Aseptically add 10.0mL of sterile glucose so-
lution. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use:  For the cultivation and maintenance of Flexibacter species.