山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Flexibacter Medium
(ATCC Medium 1559)

培養(yǎng)基配方
Composition per liter:
Solution B.............................................................700.0mL
Solution A.............................................................300.0mL
Solution A:
Composition per 300.0mL:
Pancreatic digest of casein..............................................0.5g
Yeast extract............................................................0.5g
Beef extract.............................................................0.2g
Sodium acetate...........................................................0.2g
Preparation of Solution A: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C.
Solution B:
Aged seawater.........................................................700.0mL
Preparation of Solution B: Allow seawater to sit for 7 days. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Preparation of Medium:  Aseptically add 300.0mL of sterile solu-
tion A to 700.0mL of sterile solution B at 45°–50°C. Mix thoroughly.
Aseptically distribute into sterile tubes or flasks.
Use:  For the cultivation of Flexibacter maritimus.