- 定做培養(yǎng)基/定制培養(yǎng)基
- 顆粒培養(yǎng)基
- 標(biāo)準(zhǔn)菌株生化鑒定試劑盒
- 預(yù)灌裝即用型成品培養(yǎng)基
- 2025年版中國(guó)藥典
- 促銷/特價(jià)商品
- 院感/疾控/體外診斷/采樣管
- 樣品采集與處理(均質(zhì))產(chǎn)品
- 按標(biāo)準(zhǔn)檢索培養(yǎng)基
- 模擬灌裝用培養(yǎng)基
- 干燥粉末培養(yǎng)基
- 培養(yǎng)基添加劑/補(bǔ)充劑
- 生化反應(yīng)鑒定管
- 染色液等配套產(chǎn)品
- 對(duì)照培養(yǎng)基/標(biāo)準(zhǔn)品
- 實(shí)驗(yàn)耗材與器具
- 生化試劑/化學(xué)試劑
- 菌種鑒定服務(wù)
培養(yǎng)基配方
您現(xiàn)在的位置： 網(wǎng)站首頁 >> 培養(yǎng)基配方
PGT Medium
[所屬分類:培養(yǎng)基配方] [發(fā)布時(shí)間:2021-7-5] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]
PGT Medium
L- Glutamic acid.............................................................................0.5g
MgSO 4 ·7H 2 O..............................................................................0.45g
Maltose .........................................................................................0.2g
L- Cystine.......................................................................................0.2g
DL- Tryptophan...............................................................................0.1g
Solution 3...............................................................................100.0mL
Solution 2...................................................................................2.0mL
Calcium pantothenate (0.1% solution) ......................................0.5mL
pH 6.8 ± 0.2 at 25°C
Solution 3:
Composition per 500.0mL:
Maltose .....................................................................................200.0g
CaCl 2 .............................................................................................1.5g
Calcium pantothenate (0.1% solution) ......................................3.0mL
FeSO 4 (1% in 1N HCl) ..............................................................0.2mL
Preparation of Solution 3: Add components to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15
min at 7 psi pressure–111°C. Cool to 45°–50°C.
Solution 2:
Composition per 100.0mL:
β-Alanine.................................................................................. 0.115g
Nicotinic acid............................................................................ 0.115g
CuSO 4 ·5H 2 O...............................................................................0.05g
ZnSO 4 ·7H 2 O.............................................................................0.045g
MnCl 2 ·4H 2 O .............................................................................0.015g
Pimelic acid ...............................................................................7.5mg
HCl, concentrated......................................................................3.0mL
Preparation of Solution 2: Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Add components, except solution 3, to dis-
tilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Ad-
just pH to 6.8 with 50% KOH. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add
sterile solution 3. Mix thoroughly. Aseptically distribute into sterile tubes
or flasks.
Use: For the cultivation of Corynebacterium diphtheriae.
山東拓普生物工程有限公司 培養(yǎng)基配方 http://www.jinhuabanruo.cn
Composition per liter:
Casamino acids...........................................................................30.0gL- Glutamic acid.............................................................................0.5g
MgSO 4 ·7H 2 O..............................................................................0.45g
Maltose .........................................................................................0.2g
L- Cystine.......................................................................................0.2g
DL- Tryptophan...............................................................................0.1g
Solution 3...............................................................................100.0mL
Solution 2...................................................................................2.0mL
Calcium pantothenate (0.1% solution) ......................................0.5mL
pH 6.8 ± 0.2 at 25°C
Solution 3:
Composition per 500.0mL:
Maltose .....................................................................................200.0g
CaCl 2 .............................................................................................1.5g
Calcium pantothenate (0.1% solution) ......................................3.0mL
FeSO 4 (1% in 1N HCl) ..............................................................0.2mL
Preparation of Solution 3: Add components to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15
min at 7 psi pressure–111°C. Cool to 45°–50°C.
Solution 2:
Composition per 100.0mL:
β-Alanine.................................................................................. 0.115g
Nicotinic acid............................................................................ 0.115g
CuSO 4 ·5H 2 O...............................................................................0.05g
ZnSO 4 ·7H 2 O.............................................................................0.045g
MnCl 2 ·4H 2 O .............................................................................0.015g
Pimelic acid ...............................................................................7.5mg
HCl, concentrated......................................................................3.0mL
Preparation of Solution 2: Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Add components, except solution 3, to dis-
tilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Ad-
just pH to 6.8 with 50% KOH. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add
sterile solution 3. Mix thoroughly. Aseptically distribute into sterile tubes
or flasks.
Use: For the cultivation of Corynebacterium diphtheriae.
