Horikoshi-1 Medium with 10% Sodium Chloride

(DSMZ Medium 1081a)

培養(yǎng)基配方

Composition per liter:
NaCl..........................................................................................100.0g
Agar............................................................................................15.0g
Glucose ......................................................................................10.0g
Polypeptone ..................................................................................5.0g
Yeast extract .................................................................................5.0g
KH 2 PO 4 ........................................................................................1.0g
K 2 HPO 4 ........................................................................................1.0g
MgSO 4 · 7H 2 O................................................................................0.2g
NaCO 3 solution.......................................................................100.0mL
pH 10.0 ± 0.2 at 25°C
NaCO 3 Solution :
Composition per 100.0mL:
NaCO 3 .........................................................................................10.0g
Preparation of NaCO 3 Solution:  Add NaCO 3 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except NaCO 3 solution, to double distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Adjust pH to 10.0. Gently heat while stirring and bring to boiling. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add 100.0mL NaCO 3 solution. Adjust pH to 10.0. Pour into Petri dishes or aseptically distribute into tubes.
Use: For the cultivation of Salinicoccus alkaliphilus.