Hofer’s Alkaline Medium

培養(yǎng)基配方

Composition per liter:
Agar............................................................................................15.0g
Mannitol......................................................................................10.0g
Yeast extract..................................................................................1.0g
K 2 HPO 4 .........................................................................................0.5g
MgSO4..........................................................................................0.2g
NaCl..............................................................................................0.1g
Thymol Blue.............................................................................0.016g
pH 11.0 ± 0.2 at 25°C

Source: This medium is available from HiMedia. 

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the selective isolation of Agrobacterium spp. from soil.