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Yeast Synthetic Minimal Medium
[所屬分類:培養(yǎng)基配方] [發(fā)布時(shí)間:2021-12-29] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]
Yeast Synthetic Minimal Medium
山東拓普生物工程有限公司 培養(yǎng)基配方 http://www.jinhuabanruo.cn
Composition per liter:
Glucose .............................................................20.0g
Agar ..................................................................15.0g
Yeast nitrogen base without amino acids............6.7g
Preparation of Medium: Add glucose and yeast
nitrogen base without amino acids to distilled/deion
ized water and bring volume to 100.0mL. Mix thor
oughly. Filter sterilize. Add agar to distilled/
deionized water and bring volume to 900.0mL. Mix
thoroughly. Gently heat and bring to boiling. Auto
clave for 15 min at 15 psi pressure–121°C. Cool to
50°C. Aseptically combine the two sterile solutions.
Mix thoroughly. Pour into sterile Petri dishes or asep
tically distribute into sterile tubes.
Use: For the cultivation and maintenance of Pichia an
gusta and Saccharomyces cerevisiae.
